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An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
This is also known as inoculation, spawning or adding spawn. Its main advantages are to reduce chances of contamination while giving mushrooms a firm beginning. [3] [4] Definition of Spawn: Spawn is a type of medium present in mushroom tissue that propagates the fungus such as Trichoderma which is the root system of mushrooms. [5]
The inoculation loop is first sterilized by passing it through a flame. When the loop is cool, it is dipped into an inoculum such as a broth or patient specimen containing many species of bacteria. The inoculation loop is then dragged across the surface of the agar back and forth in a zigzag motion until approximately 30% of the plate has been ...
A close up of an inoculation needle. An inoculation needle is a laboratory equipment used in the field of microbiology to transfer and inoculate living microorganisms. [1] [full citation needed] It is one of the most commonly implicated biological laboratory tools and can be disposable or re-usable. [1]
Another way of plating organisms, next to streaking, on agar plates is the spot analysis. This type of analysis is often used to check the viability of cells and is performed with pinners (often also called froggers). A third technique is using sterile glass beads to plate out cells.
Life cycle of corn grey leaf spot. Cercospora zeae-maydis survives only as long as infected corn debris is present; however, it is a poor soil competitor. The debris on the soil surface is a cause for primary inoculation that infects the incoming corn crop for the next season.
In microbiology, the multiplicity of infection or MOI is the ratio of agents (e.g. phage or more generally virus, bacteria) to infection targets (e.g. cell).For example, when referring to a group of cells inoculated with virus particles, the MOI is the ratio of the number of virus particles to the number of target cells present in a defined space.
Fungal inoculation alone can benefit host plants. Inoculation paired with other amendments can further improve conditions. Arbuscular mycorrhizal inoculation combined with compost is a common household amendment for personal gardens, agriculture, and nurseries.