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The Phadebas Forensic Press test is used as a presumptive test for saliva. [5] Phadebas may be used to find saliva as a DNA source, or to identify the origin of a stain. The test is performed by placing paper bound with the Phadebas substrate to a sample, and applying pressure. [5]
An anti-α-amylase antibody is fixed at the test line. If human salivary amylase is present in the sample, it will form an antigen-antibody-colloidal gold complex upon deposition. These complexes react with the antibodies fixed at the test line to form a visible red line indicating the presence of human salivary α-amylase, and therefore, human ...
Clinistrips quantitatively test for sugar in urine; The Kastle-Meyer test tests for the presence of hemoglobin; Salicylate testing is a category of drug testing that is focused on detecting salicylates such as acetylsalicylic acid for either biochemical or medical purposes. The Phadebas test tests for the presence of saliva for forensic purposes
A presumptive test to detect saliva is the alpha-amylase test also known as the Phadebas Test. [4] This detection technique is based on the activity of the enzyme alpha-amylase which breaks down starches from food into smaller oligosaccharide molecules, starting digestion in the mouth. [ 11 ]
The falling number method is uncomplicated, but requires an apparatus which follows the international standards. Such an apparatus consists of a water bath, a test tube, a stirring rod, and a stirring device. The test was performed manually when first employed, test instrumentation today is mostly automated. Flour samples can be analyzed directly.
The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate test. The lower case "i" is merely for "in" as the Citrate test requires coliform samples to be placed "in Citrate". These tests are useful in distinguishing members of ...
A common protocol used in the past for zymography of α-amylase activity was the so-called starch film protocol of W.W. Doane. Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or more properly, suspended) in it was overlaid for a period of time on top of the original gel. [6]
The starch iodine test, a development of the iodine test, is based on colour change, as α-amylase degrades starch and is commonly used in many applications. A similar but industrially produced test is the Phadebas amylase test, which is used as a qualitative and quantitative test within many industries, such as detergents, various flour, grain ...