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A condenser (right) and its respective diaphragm (left) A condenser is an optical lens that renders a divergent light beam from a point light source into a parallel or converging beam to illuminate an object to be imaged. Condensers are an essential part of any imaging device, such as microscopes, enlargers, slide projectors, and telescopes.
Nine-blade iris Pentacon 2.8/135 lens with 15-blade iris Aperture mechanism of Canon 50mm f/1.8 II lens, with five blades In the human eye, the iris (light brown) acts as the diaphragm and continuously constricts and dilates its aperture (the pupil) A 750nm titanium-sapphire laser beam passing through an iris diaphragm, while opening and closing the iris.
A bright-field microscope has many important parts including; the condenser, the objective lens, the ocular lens, the diaphragm, and the aperture. Some other pieces of the microscope that are commonly known are the arm, the head, the illuminator, the base, the stage, the adjusters, and the brightness adjuster.
The field diaphragm is then partially closed; the edges of the diaphragm should be in the same conjugate image planes as the specimen, therefore should appear in focus. The focus can be adjusted by raising or lowering the condenser lenses and diaphragm. Finally, the field diaphragm is reopened to just beyond the field of view.
Different apertures of a lens In biology, the pupil (appearing as a black hole) of the eye is its aperture and the iris is its diaphragm. In humans, the pupil can constrict to as small as 2 mm (f / 8.3) and dilate to larger than 8 mm (f / 2.1) in some individuals.
For Köhler illumination the light source and condenser diaphragm should appear in focus at the back focal plane of the objective lens. For phase contrast microscopy the phase ring (at the back focal plane of the objective) and the annulus (at the back focal plane of the condenser lens) should appear in focus and in alignment.
The pupil function or aperture function describes how a light wave is affected upon transmission through an optical imaging system such as a camera, microscope, or the human eye. More specifically, it is a complex function of the position in the pupil [ 1 ] or aperture (often an iris ) that indicates the relative change in amplitude and phase ...
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...