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Gel electrophoresis uses a gel as an anticonvective medium or sieving medium during electrophoresis. Gels suppress the thermal convection caused by the application of the electric field and can also simply serve to maintain the finished separation so that a post electrophoresis stain can be applied. [ 3 ]
The gel piece containing the fragment is excised (cut out from the whole gel) and placed in a dialysis bag with buffer. Electrophoresis causes the DNA to migrate out of the gel into the dialysis bag buffer. The DNA fragments are recovered from this buffer and purified, using phenol–chloroform extraction followed by ethanol precipitation. This ...
Electrophoresis apparatus: used to detect and classify serum proteins or proteins from any other source; also used for DNA separation Chromatography: • Gas chromatography or Gas liquid chromatography (GLC) • Planar chromatography • Paper chromatography • Thin layer chromatography • Affinity chromatography • Ion exchange chromatography
An example Gel documentation system, showing the results of gel electrophoresis on a connected monitor.. A gel doc, also known as a gel documentation system, gel image system or gel imager, refers to equipment widely used in molecular biology laboratories for the imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
The concentration is measured in weight of agarose over volume of buffer used (g/ml). For a standard agarose gel electrophoresis, a 0.8% gel gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel gives good resolution for small 0.2–1kb fragments. 1% gels is often used for a standard electrophoresis. [25]
Agarose gel Tray with a stack consisting top down of a weight, paper towels, membrane of nitrocellulose or nylon, gel, salt solution and a slab of glass. Southern blot membrane after hybridization and rinsing. Southern blot agarose gel under ultraviolet illumination. Southern blot autoradiogram.
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