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Anaerobic bacteria can be divided into strict anaerobes that can not grow in the presence of more than 0.5% oxygen and moderate anaerobic bacteria that are able of growing between 2 and 8% oxygen. [1] Anaerobic bacteria usually do not possess catalase, but some can generate superoxide dismutase which protects them from oxygen.
Clostridium species are endospore-forming bacteria, and can survive in atmospheric concentrations of oxygen in this dormant form. The remaining bacteria listed do not form endospores. [5] Several species of the Mycobacterium, Streptomyces, and Rhodococcus genera are examples of obligate anaerobe found in soil. [10]
A facultative anaerobic organism is an organism that makes ATP by aerobic respiration if oxygen is present, but is capable of switching to fermentation if oxygen is absent. [1] [2] Some examples of facultatively anaerobic bacteria are Staphylococcus spp., [3] Escherichia coli, Salmonella, Listeria spp., [4] Shewanella oneidensis and Yersinia ...
A number of techniques are employed by microbiologists when culturing anaerobic organisms, for example, handling the bacteria in a glovebox filled with nitrogen or the use of other specially sealed containers, or techniques such as injection of the bacteria into a dicot plant, which is an environment with limited oxygen.
Unlike obligate anaerobes however, they are not poisoned by oxygen. They can be found evenly spread throughout the test tube. Aerotolerant anaerobes use fermentation to produce ATP. They do not use oxygen, but they can protect themselves from reactive oxygen molecules. In contrast, obligate anaerobes can be harmed by reactive oxygen molecules.
As facultative anaerobes, they do survive anaerobic conditions, but grow better with a little oxygen. [9] Magnetospirillum gryphiswaldense and Magnetospira sp. QH-2 are aquatic microaerophilic magnetotactic bacteria. The formation of magnetite in such bacteria in general require microaerobic conditions. [1]
Anaerobic organisms require an oxygen-free environment. When culturing anaerobic microbes, broths are often flushed with nitrogen gas to extinguish oxygen present, and growth can also occur on media in a chamber without oxygen present. [1] Sodium resazurin can be added to indicate redox potential. [2]
Aerotolerant anaerobes do not use oxygen but are not harmed by it. [6] When an organism is able to survive in both oxygen and anaerobic environments, the use of the Pasteur effect can distinguish between facultative anaerobes and aerotolerant organisms. If the organism is using fermentation in an anaerobic environment, the addition of oxygen ...