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Due to the presence of chlorophyll a, as opposed to bacteriochlorophyll, Photosystem II absorbs light at a shorter wavelength. The pair of chlorophyll molecules at the reaction center are often referred to as P680. [1] When the photon has been absorbed, the resulting high-energy electron is transferred to a nearby pheophytin molecule.
The light-harvesting system of PSI uses multiple copies of the same transmembrane proteins used by PSII. The energy of absorbed light (in the form of delocalized, high-energy electrons) is funneled into the reaction center, where it excites special chlorophyll molecules (P700, with maximum light absorption at 700 nm) to a higher energy level.
It absorbs most energy from wavelengths of violet-blue and orange-red light, and it is a poor absorber of green and near-green portions of the spectrum. [3] Chlorophyll does not reflect light but chlorophyll-containing tissues appear green because green light is diffusively reflected by structures like cell walls. [4]
Conversely, it is a poor absorber of green and near-green portions of the spectrum. Hence chlorophyll-containing tissues appear green because green light, diffusively reflected by structures like cell walls, is less absorbed. [1] Two types of chlorophyll exist in the photosystems of green plants: chlorophyll a and b. [6]
P680 receives excitation energy either by directly absorbing a photon of suitable frequency or indirectly from other chlorophylls within photosystem II, thereby exciting an electron to a higher energy level. The resulting P680 with a loosened electron is designated as P680*, which is a strong reducing agent.
Chlorophyll fluorescence can be used as a proxy of plant stress because environmental stresses, e.g. extremes of temperature, light and water availability, can reduce the ability of a plant to metabolise normally. This can mean an imbalance between the absorption of light energy by chlorophyll and the use of energy in photosynthesis. [11]
An overview of absorption of electromagnetic radiation.This example shows the general principle using visible light as a specific example. A white light source—emitting light of multiple wavelengths—is focused on a sample (the pairs of complementary colors are indicated by the yellow dotted lines).
Chlorophyll b is a form of chlorophyll. Chlorophyll b helps in photosynthesis by absorbing light energy. It is more soluble than chlorophyll a in polar solvents because of its carbonyl group. Its color is green, and it primarily absorbs blue light. [2] In land plants, the light-harvesting antennae around photosystem II contain the majority of ...