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In 2007, Clontech was involved in litigation with Invitrogen over patents for RNase H minus reverse transcriptase. [ 6 ] Subsidiaries of Takara Bio Inc. include Takara Bio USA (formerly Clontech Laboratories), a Mountain View, California -based manufacturer of kits, reagents, instruments, and services for biological research, as well as ...
The Qubit fluorometer is a laboratory instrument developed and distributed by Invitrogen, which is now a part of Thermo Fisher. It is used for the quantification of DNA, RNA, and protein. [1] [2] [3] [4]
The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology . [ 1 ]
Invitrogen was founded in 1987 by Lyle Turner, Joe Fernandez, and William McConnell and was incorporated in 1989. The company initially found success with its kits for molecular cloning—notably, The Librarian, a kit for making cDNA libraries, and the FastTrack Kit for mRNA isolation from biological samples.
In 2001, the Applied Biosystems division of Applera reached revenues of US$1.6 billion, [25] and developed a new workstation instrument specifically for the new field of proteomics, which had become Celera's new core business focus, as it shifted away from gene discovery. The instrument analyzed 1,000 protein samples per hour. [1]
The first step in Gateway cloning is the preparation of a Gateway Entry clone. There are a few different ways to make entry clone. Gateway attB1 and attB2 sequences are added to the 5' and 3' end of a gene fragment, respectively, using gene-specific PCR primers and PCR amplification. The PCR amplification products are then mixed with a propriet
Pioneers of voltage-sensitive dyes: A. Grinvald, L.B. Cohen, K. Kamino, B.M. Salzberg, W.N. Ross; Tokyo, 2000. Fast-response probes: These are amphiphilic membrane staining dyes which usually have a pair of hydrocarbon chains acting as membrane anchors and a hydrophilic group which aligns the chromophore perpendicular to the membrane/aqueous interface.
Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. [2] Spectrophotometry uses photometers , known as spectrophotometers, that can measure the intensity of a light beam at different wavelengths.
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