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  2. Buffered charcoal yeast extract agar - Wikipedia

    en.wikipedia.org/wiki/Buffered_charcoal_yeast...

    Slowly, add enough 1.0 N KOH (about 40 mL) to the buffer solution to bring the pH up to 6.9 and mix. (Do not use NaOH because it has been found to be inhibitory to Legionella pneumophila). Into a second flask, add charcoal, yeast extract, alpha-keto-glutarate, and agar. Mix the dry powders.

  3. Buffer solution - Wikipedia

    en.wikipedia.org/wiki/Buffer_solution

    A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. [1] Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical ...

  4. TE buffer - Wikipedia

    en.wikipedia.org/wiki/TE_buffer

    10 mM Tris, bring to pH 8.0 with HCl; 1 mM EDTA, bring to pH 8.0 with NaOH; TE buffer is also known as T 10 E 1 buffer, which can be read as "T ten E one buffer". To make a 100 ml solution of T 10 E 1 buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter ...

  5. pH - Wikipedia

    en.wikipedia.org/wiki/PH

    The pH range is commonly given as zero to 14, but a pH value can be less than 0 for very concentrated strong acids or greater than 14 for very concentrated strong bases. [2] The pH scale is traceable to a set of standard solutions whose pH is established by international agreement. [3]

  6. Magic acid - Wikipedia

    en.wikipedia.org/wiki/Magic_acid

    Magic acid (FSO 3 H·SbF 5) is a superacid consisting of a mixture, most commonly in a 1:1 molar ratio, of fluorosulfuric acid (HSO 3 F) and antimony pentafluoride (SbF 5).This conjugate Brønsted–Lewis superacid system was developed in the 1960s by Ronald Gillespie and his team at McMaster University, [1] and has been used by George Olah to stabilise carbocations and hypercoordinated ...

  7. Alkalinity - Wikipedia

    en.wikipedia.org/wiki/Alkalinity

    The lower the pH, the higher the concentration of bicarbonate will be. This shows how a lower pH can lead to higher alkalinity if the amount of bicarbonate produced is greater than the amount of H + remaining after the reaction. This is the case since the amount of acid in the rainwater is low.