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Winged elbow pipetting Technique: elevated, “winged elbow”. The average human arm weighs approximately 6% of the total body weight. Holding a pipette with the elbow extended (winged elbow) in a static position places the weight of the arm onto the neck and shoulder muscles and reduces blood flow, thereby causing stress and fatigue.
Forward pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for aqueous solutions, such as buffers, or diluted acids or alkalis. In case of solutions with a high viscosity or a tendency to foam, reverse pipetting is more suitable.
Reverse pipetting is a technique to dispense a measured quantity of liquid by means of air displacement pipette. The technique is mainly recommended for solutions with a high viscosity or a tendency to foam: [ 1 ] as it reduces the risk of splashing, foam or bubble formation.
In developmental, cell and molecular biology, trituration is the process of fragmenting of solid material (often biological tissue or aggregated material) into smaller components (often, respectively, cells or molecules in suspension/solution) by means of repeated passage through a pipette.
Whereas manual pipettes need a movement of the thumb (up to 3 cm), electronic pipettes have a main button. The programming of the pipette is generally done by a control wheel and some further buttons. All settings are displayed on a small display. Electronic pipettes can decrease the risk of RSI-type injuries. [8] [9]
Positive displacement pipettes are a type of pipette that operates via piston-driven displacement. [1] [2] Unlike an air displacement pipette, which dispenses liquid using an air cushion in the pipette tip, the piston in a positive displacement pipette makes direct contact with the sample, allowing the aspiration force to remain constant.
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate.
A Mohr pipette, also known as a graduated pipette, is a type of pipette used to measure the volume of the liquid dispensed, although not as accurately as a volumetric pipette. [1] These use a series of marked lines (as on a graduated cylinder) to indicate the different volumes. [ 2 ]