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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Micropropagation or tissue culture is the practice of rapidly multiplying plant stock material to produce many progeny plants, using modern plant tissue culture methods. [ 1 ] Micropropagation is used to multiply a wide variety of plants, such as those that have been genetically modified or bred through conventional plant breeding methods.
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. [1] This is possible only in certain conditions. It also requires more attention.
In tissue culture, plant cells are taken from various parts of the plant and are cultured and nurtured in a sterilized medium. The mass of developed tissue, known as the callus , is then cultured in a hormone-ladened medium and eventually develops into plantlets which are then planted and eventually develop into grown plants.
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The lifespan of most cells is genetically determined, but some cell-culturing cells have been 'transformed' into immortal cells which will reproduce indefinitely if the optimal conditions are provided.
The substance used to embed tissue is embedding media, which is chosen depends on the category of the microscope, category of the micro tome, and category of tissue. [23] Paraffin wax, whose melting point is from 56 to 62°C, is commonly used for embedding. [22] Tissue processing - Tissue sections on slides are stained on an automated stainer
Agricultural biotechnology, also known as agritech, is an area of agricultural science involving the use of scientific tools and techniques, including genetic engineering, molecular markers, molecular diagnostics, vaccines, and tissue culture, to modify living organisms: plants, animals, and microorganisms. [1]
A light-dependent reaction was observed in that a decrease in the available CO 2 was found after the light switch in sealed containers used in conventional tissue culture. [2] This led to the idea that plants, even in vitro, are capable of actively producing sugars via photosynthesis and this idea was quickly tested and validated using potato ...