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The accuracy of serologic testing has been verified by isolation and culture of HIV and by detection of HIV RNA by PCR, which are widely accepted "gold standards" in microbiology. [ 23 ] [ 24 ] While AIDS denialists focus on individual components of HIV testing, the combination of ELISA and western blot used for the diagnosis of HIV is ...
However, the accuracy of serologic testing has in fact been verified by isolation and culture of HIV and by detection of HIV RNA by PCR; these are widely accepted "gold standards" in microbiology.[18][19] While the AIDS dissidents focused on individual components of HIV testing, the combination of ELISA and Western Blot used for the diagnosis ...
The window period for HIV may be up to three months, depending on the test method and other factors. RNA based HIV tests has the lowest window period. Modern and accurate testing abilities can cut this period to 25 days, 16 days, or even as low as 12 days, again, depending on the type of test and the quality of its administration and interpretation.
Pooled viral RNA testing shortens the window period to a median of 17 days (95% CI, 13-28 Days). [9] Although it is not the standard of care to use this test for diagnosis, in communities with high HIV prevalence, this test has a significantly improved negative predictive value over 3rd and 4th generation tests for detecting acute HIV infections.
The latest recommendations of the US Centers for Disease Control and Prevention (CDC) show that HIV testing must start with an immunoassay combination test for HIV-1 and HIV-2 antibodies and p24 antigen. A negative result rules out HIV exposure, while a positive one must be followed by an HIV-1/2 antibody differentiation immunoassay to detect ...
Thus HIV infection can only be diagnosed by PCR testing for HIV RNA or DNA, or via testing for the p24 antigen. [30] Much of the world lacks access to reliable PCR testing, and people in many places simply wait until either symptoms develop or the child is old enough for accurate antibody testing. [113]
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