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However, in FLIP, multiple photobleaching events occur to prevent the return of unbleached fluorophores to the bleaching region. Like FLIP, FRAP is used in the study of continuity of membranous organelles. FLIP and FRAP are often used together to determine the mobility of GFP-tagged proteins. [3]
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
A biological network is a method of representing systems as complex sets of binary interactions or relations between various biological entities. [1] In general, networks or graphs are used to capture relationships between entities or objects. [1]
To study structures within living cells, in combination with fluorescence, one can utilize recombinant proteins containing fluorescent protein domains, e.g., green fluorescent protein (GFP). The GFP-technique involves altering the genetic information of the cells.
Biological network inference is the process of making inferences and predictions about biological networks. [1] By using these networks to analyze patterns in biological systems, such as food-webs, we can visualize the nature and strength of these interactions between species, DNA, proteins, and more.
Biology Today is a college-level biology textbook that went through three editions in 1972, 1975, and 1980. The first edition, published by Communications Research Machines, Inc. (CRM) and written by a small editorial team and large set of prominent "contributing consultants", is notable for its lavish illustrations and its humanistic approach.
Biology Today: An Issues Approach is a college-oriented Biology textbook by Eli C. Minkoff and Pamela J. Baker designed to integrate the teaching of biological concepts within the context of current societal issues relating to these topics. [1] It is the original issues-oriented introductory-level general biology textbook. [2]
The DNA double helix with a cytosine base flipped out 180° DNA base flipping, or nucleotide flipping, is a mechanism in which a single nucleotide base, or nucleobase, is rotated outside the nucleic acid double helix. [1]