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Field-flow fractionation, abbreviated FFF, [1] is a separation technique invented by J. Calvin Giddings. The technique is based on separation of colloidal or high molecular weight substances in liquid solutions, flowing through the separation platform, which does not have a stationary phase .
Asymmetrical flow field flow fractionation (AF4) is nowadays a common and state-of-the art method for fractionation and separation of macromolecules and particles in a suspension. AF4 is an alternative to HPLC and SEC in cases where column chromatography is not suitable for the analyte.
Eventually Prof Giddings invented the one-phase separation technique called "field-flow fractionation" (FFF). [17] [18] [19] He developed the concept of a chromatographic-like system in which retention is established and controlled by an external field rather than by the stationary phase. The FFF system extends the range of chromatography ...
Polymers can be fractionated on an analytical scale by size exclusion chromatography (SEC), Matrix-assisted laser desorption/ionization (MALDI) or field flow fractionation (FFF). These methods are used to determine the molecular weight distribution.
Dielectrophoresis field-flow fractionation (DEP-FFF), introduced by Davis and Giddings, [42] is a family of chromatographic-like separation methods. In DEP-FFF, DEP forces are combined with drag flow to fractionate a sample of different types of particles.
Fractionation makes it possible to isolate more than two components in a mixture in a single run. This property sets it apart from other separation techniques. Fractionation is widely employed in many branches of science and technology. Mixtures of liquids and gasses are separated by fractional distillation by difference in boiling point.
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Sedimentation field flow fractionation (SFFF) is a non-destructive separation technique which can be used for both separation, and collecting fractions. Some ...