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Parfocal microscope objectives stay in focus when magnification is changed; i.e., if the microscope is switched from a lower power objective (e.g., 10×) to a higher power objective (e.g., 40×), the object stays in focus. Most modern bright-field microscopes are parfocal.
Bright-field microscopy (BF) is the simplest of all the optical microscopy illumination techniques. Sample illumination is transmitted (i.e., illuminated from below and observed from above) white light, and contrast in the sample is caused by attenuation of the transmitted light in dense areas of the sample.
In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
Medical microbiology. A microbiologist examining cultures under a dissecting microscope. Medical microbiology, the large subset of microbiology that is applied to medicine, is a branch of medical science concerned with the prevention, diagnosis and treatment of infectious diseases. In addition, this field of science studies various clinical ...
Optical microscope. The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Micrograph showing contraction band necrosis, a histopathologic finding of myocardial infarction (heart attack).. Histopathology (compound of three Greek words: ἱστός histos 'tissue', πάθος pathos 'suffering', and -λογία-logia 'study of') is the microscopic examination of tissue in order to study the manifestations of disease.
A zoom lens is a system of camera lens elements for which the focal length (and thus angle of view) can be varied, as opposed to a fixed-focal-length (FFL) lens (prime lens). A true zoom lens or optical zoom lens is a type of parfocal lens, one that maintains focus when its focal length changes. [1] Most consumer zoom lenses do not maintain ...
Endomicroscopy. Endomicroscopy is a technique for obtaining histology -like images from inside the human body in real-time, [1][2][3] a process known as ‘optical biopsy’. [4][5] It generally refers to fluorescence confocal microscopy, although multi-photon microscopy and optical coherence tomography have also been adapted for endoscopic use ...