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Plasmid miniprep. 0.8% agarose gel ethidium bromide-stained.. A plasmid preparation is a method of DNA extraction and purification for plasmid DNA.It is an important step in many molecular biology experiments and is essential for the successful use of plasmids in research and biotechnology.
Due to the use of kits with multiplex amplification, it is necessary to have a lower amount of EDTA in the sample so as not to interfere with the Mg 2+ present in the reaction. If regular TE buffer is used to dilute the sample, an imbalance will be observed in the DNA profile, while Low TE will have a better balance.
The MerckManual and MerckHome templates helps markup links to respectively the Professional Edition and Home Edition of the Merck Manual of Diagnosis and Therapy medical textbooks. Three parameters are required, with options (to maintain backwards compatibility) for a fourth for page number and fifth for alternative displayed text. Section number
Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Electrophoretic mobility is a function of the length, conformation, and ...
Taq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by master's student Alice Chien et al. in 1976. [1]
A first aid kit or medical kit is a collection of supplies and equipment used to give immediate medical treatment, primarily to treat injuries and other mild or moderate medical conditions. There is a wide variation in the contents of first aid kits based on the knowledge and experience of those putting it together, the differing first aid ...