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RNA-dependent RNA polymerase (RdRp) or RNA replicase is an enzyme that catalyzes the replication of RNA from an RNA template. Specifically, it catalyzes synthesis of the RNA strand complementary to a given RNA template.
NTP binding sites play a role in poliovirus RNA replication. [1] On the poliovirus RNA-dependent polymerase, also known as 3D pol, there are two binding sites. Both binding sites contain lysine residues; however, only the lysine at position 61 is essential for RNA sequence elongation as part of the replication process. [2]
The first Mg 2+ will hold on to the α-phosphate of the NTP to be added. This allows the nucleophilic attack of the 3′-OH from the RNA transcript, adding another NTP to the chain. The second Mg 2+ will hold on to the pyrophosphate of the NTP. [19] The overall reaction equation is: (NMP) n + NTP → (NMP) n+1 + PP i
RNA polymerase, assisted by one or more general transcription factors, then selects a transcription start site in the transcription bubble, binds to an initiating NTP and an extending NTP (or a short RNA primer and an extending NTP) complementary to the transcription start site sequence, and catalyzes bond formation to yield an initial RNA ...
Nucleic acid synthesis is catalyzed by either DNA polymerase or RNA polymerase for DNA and RNA synthesis respectively. [16] These enzymes covalently link the free -OH group on the 3’ carbon of a growing chain of nucleotides to the α-phosphate on the 5’ carbon of the next (d)NTP, releasing the β- and γ-phosphate groups as pyrophosphate ...
The usage of RNA primers during DNA replication is an example of a correct incorporation of rNTPs during the process. Although, overly long RNA primers can decrease the effectiveness of T7 DNA polymerase in incorporating dNTP into the growing strand and weaken the binding between T7 and the template DNA strand.
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Without the need of a primer, RNA polymerase can initiate the synthesis of a new RNA chain using the template DNA strand to guide ribonucleotide selection and polymerization chemistry. [1] However, many of the initiated syntheses are aborted before the transcripts reach a significant length (~10 nucleotides).