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The analytical profile index (API) was invented in the 1970s in the United States by Pierre Janin of Analytab Products Inc. [2] The API test system is currently manufactured by bioMérieux. [3] The API range introduced a standardized and miniaturized version of existing techniques, which were considered complicated to perform and difficult to read.
For example, Salmonella enteritidis would yield a positive (blue) result on Simmons’ agar and thus be distinguished from other Salmonella species like Salmonella typhi, Salmonella pullorum, and Salmonella gallinarum, which would yield a negative (green) result.
The Kauffmann–White classification or Kauffmann and White classification scheme [1] [2] is a system that classifies the genus Salmonella into serotypes, based on surface antigens. It is named after Philip Bruce White and Fritz Kauffmann . First the "O" antigen type is determined based on oligosaccharides associated with lipopolysaccharide.
Enterobacteriaceae is a large family of Gram-negative bacteria.It includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in the phylum Pseudomonadota.
When P. vulgaris is tested using the API 20E identification system [1] it produces positive results for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing strip.
TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar; however, the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus spp. and other non-Salmonellae. [1]
Some infections caused by K. aerogenes result from specific antibiotic treatments, venous catheter insertions, and/or surgical procedures. It is generally found in the human gastrointestinal tract and does not generally cause disease in healthy individuals. It has been found to live in various wastes, hygiene chemicals, and soil.
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