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Data erasure (sometimes referred to as data clearing, data wiping, or data destruction) is a software-based method of data sanitization that aims to completely destroy all electronic data residing on a hard disk drive or other digital media by overwriting data onto all sectors of the device in an irreversible process. By overwriting the data on ...
In immunology, clonal deletion is the process of removing T and B lymphocytes from the immune system repertoire. [1] [2] The process of clonal deletion helps prevent recognition and destruction of the self host cells, making it a type of negative selection. Ultimately, clonal deletion plays a role in central tolerance. [3]
In genetics, a deletion (also called gene deletion, deficiency, or deletion mutation) (sign: Δ) is a mutation (a genetic aberration) in which a part of a chromosome or a sequence of DNA is left out during DNA replication.
A conditional gene knockout allows gene deletion in a tissue in a tissue specific manner. This is required in place of a gene knockout if the null mutation would lead to embryonic death, [13] or a specific tissue or cell type is of specific interest. This is done by introducing short sequences called loxP sites around the gene.
Cellular deconvolution algorithms have been applied to a variety of samples collected from saliva, [5] buccal, [5] cervical, [5] PBMC, [6] brain, [2] kidney, [1] and pancreatic cells, [1] and many studies have shown that estimating and incorporating the proportions of cell types into various analyses improves the interpretability of high ...
Only a few cell types express Cre recombinase and no mammalian cells express it so there is no risk of accidental activation of lox sites when using conditional gene knockout in mammals. Figuring out how to express Cre-recombinase in an organism tends to be the most difficult part of this technique.
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The resultant gap is then filled in using DNA polymerase I and DNA ligase. The basic excision process is very similar in higher cells, but these cells usually involve many more proteins – E.coli is a simple example. [5] TC-NER also exists in bacteria, and is mediated by the TRCF (Mfd) protein.