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For example, the complete blood count can help a physician to determine why a patient feels unwell and what to do to help. Cell counts within liquid media (such as blood, plasma, lymph, or laboratory rinsate) are usually expressed as a number of cells per unit of volume, thus expressing a concentration (for example, 5,000 cells per milliliter).
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The CBC is used to determine the number or proportion of white and red blood cells in the body. Previously, this procedure involved preparing a peripheral blood smear and manually counting each type of cell under a microscope , a process that typically required a half-hour.
Instead of colony-forming units, the parameters Most Probable Number (MPN) and Modified Fishman Units (MFU) [29] can be used. The Most Probable Number method counts viable cells and is useful when enumerating low concentrations of cells or enumerating microbes in products where particulates make plate counting impractical. [30]
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The total number of living aerobic bacteria can be determined using a plate count agar which is a substrate for bacteria to grow on. The medium contains casein which provides nitrogen , carbon , amino acids , vitamins and minerals to aid in the growth of the organism.
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A small number of cells are used to inoculate parallel cultures in a non-selective medium. [11] The cultures are grown to saturation to obtain equal cell densities. The cells are plated onto selective media to obtain the number of mutants (r). Dilutions are plated onto rich medium to calculate the total number of viable cells ( N t). The number ...