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  2. Light sheet fluorescence microscopy - Wikipedia

    en.wikipedia.org/wiki/Light_sheet_fluorescence...

    The principle setup of a light sheet fluorescence microscope. Light sheet fluorescence microscopy (LSFM) is a fluorescence microscopy technique with an intermediate-to-high [1] optical resolution, but good optical sectioning capabilities and high speed.

  3. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  4. Köhler illumination - Wikipedia

    en.wikipedia.org/wiki/Köhler_illumination

    Köhler illumination is a method of specimen illumination used for transmitted and reflected light (trans- and epi-illuminated) optical microscopy.Köhler illumination acts to generate an even illumination of the sample and ensures that an image of the illumination source (for example a halogen lamp filament) is not visible in the resulting image.

  5. Structured illumination light sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Structured_Illumination...

    Structured illumination light sheet microscopy (SI-LSM) is an optical imaging technique used for achieving volumetric imaging with high temporal and spatial resolution in all three dimensions. It combines the ability of light sheet microscopy to maintain spatial resolution throughout relatively thick samples with the higher axial and spatial ...

  6. Light microscopy - Wikipedia

    en.wikipedia.org/?title=Light_microscopy&redirect=no

    Light microscopy. Add languages. Add links. Article; Talk; ... Download QR code; Print/export Download as PDF; Printable version; In other projects

  7. Polarized light microscopy - Wikipedia

    en.wikipedia.org/wiki/Polarized_light_microscopy

    Polarized light microscopy can mean any of a number of optical microscopy techniques involving polarized light. Simple techniques include illumination of the sample with polarized light. Directly transmitted light can, optionally, be blocked with a polariser oriented at 90 degrees to the illumination. More complex microscopy techniques which ...

  8. Lattice light-sheet microscopy - Wikipedia

    en.wikipedia.org/wiki/Lattice_light-sheet_microscopy

    Lattice light-sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity. This is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen, generating a time series of 3D images which can ...

  9. Bright-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Bright-field_microscopy

    The light path of a bright-field microscope is extremely simple; no additional components are required beyond the normal light-microscope setup. The light path begins at the illuminator or the light source on the base of the microscope. Often a halogen lamp is used. The light travels through the objective lens into the ocular lens, through ...