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Ruby spectra R1, R2 lines. The ruby fluorescence pressure scale is an optical method to measure pressure within a sample chamber of a diamond anvil cell apparatus. [1] Since it is an optical method, which fully make use of the transparency of diamond anvils and only requires an access to a small scale laser generator, it has become the most prevalent pressure gauge method in high pressure ...
Stopped-flow spectrophotometers may function as stand-alone instruments, but they are often integrated into systems for circular dichroism (CD), absorbance, and/or fluorescence measurements, or equipped with various accessories to support specialized applications. Common stopped-flow accessories include:
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
For example, the mass absorption coefficient of silicon at the wavelength of the aluminium Kα line is 50 m 2 /kg, whereas that of iron is 377 m 2 /kg. This means that fluorescent X-rays generated by a given concentration of aluminium in a matrix of iron are absorbed about seven times more (that is 377/50) compared with the fluorescent X-rays ...
A computer program measures the results, determining the quantity or size of the fragments, at each data point, from the level of fluorescence intensity. [1] Samples which contain higher quantities of amplified DNA will have higher corresponding RFU values. [2] [3] An "RFU peak" is a relative maximum point along a graph of the analyzed data.
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]