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Schematic diagram of the arrangement of optical components in a typical Spectrofluorometer. A spectrofluorometer is an instrument which takes advantage of fluorescent properties of some compounds in order to provide information regarding their concentration and chemical environment in a sample.
Jablonski diagram including vibrational levels for absorbance, non-radiative decay, and fluorescence. When a molecule absorbs a photon, the photon energy is converted and increases the molecule's internal energy level. Likewise, when an excited molecule releases energy, it can do so in the form of a photon.
Stopped-flow spectrophotometers may function as stand-alone instruments, but they are often integrated into systems for circular dichroism (CD), absorbance, and/or fluorescence measurements, or equipped with various accessories to support specialized applications. Common stopped-flow accessories include:
Fluorometer designed to measure chlorophyll fluorescence in plants. A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1]
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity. Its theoretical underpinning originated from L. Onsager's regression hypothesis. The analysis provides kinetic parameters of the physical processes underlying the fluctuations.
For example, if the heights of two lines are found to be h 1 and h 2, c 1 = h 1 / ε 1 and c 2 = h 2 / ε 2. [14] Parameters of the line shape are unknown. The intensity of each component is a function of at least 3 parameters, position, height and half-width. In addition one or both of the line shape function and baseline function may not be ...
This process enables it to transform the fluorescence data into a quantified concentration measurement. The device uses this established relationship to accurately determine the concentration of a sample. A specific instance of this technology is the Qubit 2.0 fluorometer, which is often used in conjunction with the "dsDNA BR Assay Kit."
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