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An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota. [1] [2] The name "endospore" is suggestive of a spore or seed-like form (endo means 'within'), but it is not a true spore (i.e., not an offspring). It is a stripped-down, dormant form to which the bacterium can reduce itself.
Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1] Within bacteria, endospores are protective structures used to survive extreme conditions, including high temperatures making them highly resistant to chemicals. [ 2 ]
A stained preparation of Bacillus subtilis showing endospores as green and the vegetative cell as red. The Schaeffer–Fulton stain is a technique designed to isolate endospores by staining any present endospores green, and any other bacterial bodies red. [1]
Fig1. The sporulation process of Bacillus subtilis. Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore.
The location of an endospore within a cell is species-specific and can be used to determine the identity of a bacterium. Dipicolinic acid is a chemical compound which composes 5% to 15% of the dry weight of bacterial spores and is implicated in being responsible for the heat resistance of endospores.
Clostridium sporogenes is a species of Gram-positive bacteria that belongs to the genus Clostridium.Like other strains of Clostridium, it is an anaerobic, rod-shaped bacterium that produces oval, subterminal endospores [2] and is commonly found in soil.
Lysinibacillus sphaericus (previously known as Bacillus sphaericus) [1] is a Gram-positive, mesophilic, rod-shaped bacterium commonly found on soil. It can form resistant endospores that are tolerant to high temperatures, chemicals and ultraviolet light and can remain viable for long periods of time.
Clostridium species are endospore-forming bacteria, and can survive in atmospheric concentrations of oxygen in this dormant form. The remaining bacteria listed do not form endospores. The remaining bacteria listed do not form endospores.