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Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance.
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
In fluorescence, the species is first excited, by absorbing a photon, from its ground electronic state to one of the various vibrational states in the excited electronic state. Collisions with other molecules cause the excited molecule to lose vibrational energy until it reaches the lowest vibrational state from the excited electronic state.
Microfluorimetry can also be used as a method to distinguish different microorganisms from one another by analyzing and comparing the DNA content of each cell. [6] This same concept can also be applied to distinguish between cell types using a suitable fluorescent dye which varies depending on purpose and is a critical technique in modern cell ...
Biofluorescence is fluorescence exhibited by a living organism: part of the organism absorbs light or other radiation at one wavelength and emits visible light at another, usually longer. The absorbed radiation is often blue or ultraviolet , while the light emitted is typically green, red, or anything in between.
Micrograph of paper autofluorescing under ultraviolet illumination. The individual fibres in this sample are around 10 μm in diameter.. Autofluorescence is the natural fluorescence of biological structures such as mitochondria and lysosomes, in contrast to fluorescence originating from artificially added fluorescent markers (fluorophores).
There was a tennis court represented by just two lines: one being the ground, the other being the net. The ball was a mere dot that bounced back and forth. And the game was unable to keep score ...
To mitigate the risk of photobleaching one can employ different strategies. By reducing or limiting the intensity, or timespan of light exposure, the absorption-emission cycle of fluorescent light is decreased, thus preserving the fluorophores functionality.