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Enterobacteria phage T2 is a virus that infects and kills E. coli. It is in the genus Tequatrovirus , and the family Myoviridae . Its genome consists of linear double-stranded DNA , with repeats at either end.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4.. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
Radioactive sulfur-35 was used to label the protein sections of the T2 phage, because sulfur is contained in protein but not DNA. [6] Hershey and Chase inserted the radioactive elements in the bacteriophages by adding the isotopes to separate media within which bacteria were allowed to grow for 4 hours before bacteriophage introduction.
The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates), Enterobacteria phage T2, Enterobacteria phage T4 and Enterobacteria phage T6. Use in research
The Hershey–Chase experiments were conducted by Alfred Hershey and Martha Chase in 1952 using the T2 bacteriophage (pictured), which is composed of DNA wrapped in a protein shell. Hershey and Chase labelled either the phage DNA using radioactive phosphorus-32 or the protein using radioactive sulphur-35 .
Bacteriophages occur in over 1100 bacterial or archaeal genera. [3] Over 6300 bacteriophages have been examined in the electron microscope since 1959. Of these, more than 96 percent have tails. Of the tailed phages, about 57 percent have long, noncontractile tails ("Siphoviridae"). Tailed phages appear to be monophyletic and are the oldest ...
Bacteriophage Lambda Structure at Atomic Resolution [1] Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. [2]
The experiment involved radioactively labeling either protein or nucleic acid of the bacteriophage T2 (a virus that infects bacteria) and seeing which component entered Escherichia coli upon infection. They found that nucleic acids but not protein were transferred, helping resolve controversy over the composition of hereditary information.