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When grown in a laboratory, Candida appears as large, round, white or cream (albicans means "whitish" in Latin) colonies, which emit a yeasty odor on agar plates at room temperature. [13] C. albicans ferments glucose and maltose to acid and gas, sucrose to acid, and does not ferment lactose, which helps to distinguish it from other Candida ...
A germ tube test is a diagnostic test in which a sample of fungal spores are suspended in animal serum and examined by microscopy for the detection of any germ tubes. [2] It is particularly indicated for colonies of white or cream color on fungal culture , where a positive germ tube test is strongly indicative of Candida albicans .
Candida albicans is an opportunistic pathogenic yeast [5] that is a common member of the human gut flora. It can also survive outside the human body. It can also survive outside the human body. [ 6 ] [ 7 ] It is detected in the gastrointestinal tract and mouth in 40–60% of healthy adults.
Gram stain of Candida albicans from a vaginal swab. The small oval chlamydospores are 2–4 μm in diameter. Gram staining is a bacteriological laboratory technique [8] used to differentiate bacterial species into two large groups (gram-positive and gram-negative) based on the physical properties of their cell walls.
The pour plate technique is the typical technique used to prepare plate count agars. Here, the inoculum is added to the molten agar before pouring the plate. The molten agar is cooled to about 45 degrees Celsius and is poured using a sterile method into a petri dish containing a specific diluted sample.
The yeast Candida albicans can live in people without producing symptoms, and is able to cause both superficial mild candidiasis in healthy people, such as oral thrush or vaginal yeast infection, and severe systemic candidiasis in those who cannot fight infection themselves. [3]
The type of medium in the agar plates is also important. Chromagar Candida is a differential medium used to identify different Candida species. When grown on this medium, C. albicans is light green, C. tropicalis is steel blue with purple around the edges, and C. krusei is rose pink with white around the edges. [7]
The time taken for a plate to be ready depends on the m that is being tested, and the conditions of the agar plate. [ citation needed ] The predefined Etest gradient remains stable for at least 18 to 24 hours; that is, a period that covers the critical times of many species of fastidious and non-fastidious organisms.