Search results
Results From The WOW.Com Content Network
These lenses are often color coded for easier use. The least powerful lens is called the scanning objective lens, and is typically a 4× objective. The second lens is referred to as the small objective lens and is typically a 10× lens. The most powerful lens out of the three is referred to as the large objective lens and is typically 40–100×.
A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution.With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.
A telecentric lens is a special optical lens (often an objective lens or a camera lens) that has its entrance or exit pupil, or both, at infinity. The size of images produced by a telecentric lens is insensitive to either the distance between an object being imaged and the lens, or the distance between the image plane and the lens, or both, and ...
So a standard 50 mm lens for 35 mm photography acts like a 50 mm standard "film" lens even on a professional digital SLR, but would act closer to a 75 mm (1.5×50 mm Nikon) or 80 mm lens (1.6×50mm Canon) on many mid-market DSLRs, and the 40-degree angle of view of a standard 50 mm lens on a film camera is equivalent to a 28–35 mm lens on ...
Field of view is the area of the inspection captured on the camera’s imager. The size of the field of view and the size of the camera’s imager directly affect the image resolution (one determining factor in accuracy). Working distance is the distance between the back of the lens and the target object.
Different kinds of camera lenses, including wide angle, telephoto and speciality. A camera lens (also known as photographic lens or photographic objective) is an optical lens or assembly of lenses (compound lens) used in conjunction with a camera body and mechanism to make images of objects either on photographic film or on other media capable of storing an image chemically or electronically.
Thus it is also called a stop (an aperture stop, if it limits the brightness of light reaching the focal plane, or a field stop or flare stop for other uses of diaphragms in lenses). The diaphragm is placed in the light path of a lens or objective , and the size of the aperture regulates the amount of light that passes through the lens.
For objective-based TIRFM, the excitation beam and fluoresced emission beam will be captured via the same objective lens. Thus, to split the beams, a dichromatic mirror is used to reflect the incoming excitation beam towards the objective lens, and allow the emission beam to pass through into the detector.