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These lenses are often color coded for easier use. The least powerful lens is called the scanning objective lens, and is typically a 4× objective. The second lens is referred to as the small objective lens and is typically a 10× lens. The most powerful lens out of the three is referred to as the large objective lens and is typically 40–100×.
In wide-field multiphoton microscopy the high intensities are best achieved using an optically amplified pulsed laser source to attain a large field of view (~100 μm). [ 2 ] [ 3 ] [ 4 ] The image in this case is obtained as a single frame with a CCD without the need of scanning, making the technique particularly useful to visualize dynamic ...
Angular field of view is typically specified in degrees, while linear field of view is a ratio of lengths. For example, binoculars with a 5.8 degree (angular) field of view might be advertised as having a (linear) field of view of 102 mm per meter. As long as the FOV is less than about 10 degrees or so, the following approximation formulas ...
Different kinds of camera lenses, including wide angle, telephoto and speciality. A camera lens (also known as photographic lens or photographic objective) is an optical lens or assembly of lenses (compound lens) used in conjunction with a camera body and mechanism to make images of objects either on photographic film or on other media capable of storing an image chemically or electronically.
Minox LX camera with hyperfocal red dot Nikon 28mm f /2.8 lens with markings for the depth of field. The lens is set at the hyperfocal distance for f /22. The orange mark corresponding to f /22 is at the infinity mark (∞). Focus is acceptable from under 0.7 m to infinity. Minolta 100–300 mm zoom lens. The depth of field, and thus hyperfocal ...
For example, 1/2.5 converts to 2/5 as a simple fraction, or 0.4 as a decimal number. This "inch" system gives a result approximately 1.5 times the length of the diagonal of the sensor. This "inch" system gives a result approximately 1.5 times the length of the diagonal of the sensor.
A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution.With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.
The sequence of lens designations may differ in literature. See also full list of Nikon designations, Sigma designations, Tamron designations and Tokina designations.. Nikon AF-S/Sigma HSM/Tamron USD or PZD: The lens contains an integrated ultrasonic focus motor based on piezoelectricity, which enables quiet or low noise autofocus operation.