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Ultramicrobacteria are bacteria that are smaller than 0.1 μm 3 under all growth conditions. [1] [2] [3] This term was coined in 1981, describing cocci in seawater that were less than 0.3 μm in diameter. [4] Ultramicrobacteria have also been recovered from soil and appear to be a mixture of gram-positive, gram-negative and cell-wall-lacking ...
Experiments generally start after 24 hours of incubation. Using living cells or tissue from the same organism are still considered to be ex vivo. One widely performed ex vivo study is the chick chorioallantoic membrane (CAM) assay. In this assay, angiogenesis is promoted on the CAM membrane of a chicken embryo outside the organism (chicken).
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Primary cell culture is the ex vivo culture of cells freshly obtained from a multicellular organism, as opposed to the culture of immortalized cell lines.In general, primary cell cultures are considered more representative of in vivo tissues than cell lines, and this is recognized legally in some countries such as the UK (Human Tissue Act 2004). [1]
In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. [1]
The first report of minicells in the scientific literature dates to 1930., [3] but the first use of the name "minicell" dates to 1967 [2] Minicells of a variety of gram negative [4] and gram positive [5] [6] bacteria, including Escherichia coli [7] and Salmonella enterica, [8] have been reported, but in principle, minicells could be generated for any bacterial species that can be genetically ...
Structures found on meteorite fragment Allan Hills 84001. Nanobacterium (/ ˌ n æ n oʊ b æ k ˈ t ɪər i əm / NAN-oh-bak-TEER-ee-əm, pl. nanobacteria / ˌ n æ n oʊ b æ k ˈ t ɪər i ə / NAN-oh-bak-TEER-ee-ə) is the unit or member name of a former proposed class of living organisms, specifically cell-walled microorganisms, now discredited, with a size much smaller than the generally ...
Obtaining a culture with a unified cell-cycle stage is useful for biological research where a particular stage in the cell cycle is desired (such as the culturing of parasitized cells [3]). Since cells are too small for certain research techniques, a synchronous culture can be treated as a single cell; the number of cells in the culture can be ...