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Amira (ah-MEER-ah) is a software platform for visualization, processing, and analysis of 3D and 4D data. It is being actively developed by Thermo Fisher Scientific in collaboration with the Zuse Institute Berlin (ZIB), and commercially distributed by Thermo Fisher Scientific — together with its sister software Avizo.
PSF Lab is a software program that allows the calculation of the illumination point spread function (PSF) of a confocal microscope under various imaging conditions. The calculation of the electric field vectors is based on a rigorous, vectorial model that takes polarization effects in the near-focus region and high numerical aperture microscope objectives into account.
Compared to most image processing libraries VIPS needs little RAM and runs quickly, especially on machines with more than one CPU. This is primarily due to its architecture which automatically parallelises the image workflows. [5] The software has two main parts: libvips is the image-processing library and nip2 is the graphical user-interface.
Several types of microscope are regularly used: widefield, confocal, or two-photon. Most microscopy system will also support the collection of time-series (movies). In general, filters are used so that each dye is imaged separately (for example, a blue filter is used to image Hoechst, then rapidly
By virtue of the linearity property of optical non-coherent imaging systems, i.e., . Image(Object 1 + Object 2) = Image(Object 1) + Image(Object 2). the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the ...
Fluorescence and confocal microscopes operating principle. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation. [1]
Transfer function and example image of an ideal, optical-aberration-free (diffraction-limited) imaging system. It can be read from the plot that the contrast gradually reduces and reaches zero at the spatial frequency of 500 cycles per millimeter, in other words the optical resolution of the image projection is 1/500 th of a millimeter, or 2 ...
Computer simulation is a computer program, or network of computers, that attempts to simulate an abstract model of a particular system. Computer simulations have become a useful part of mathematical modelling of many natural systems in physics, and computational physics, chemistry and biology; human systems in economics, psychology, and social ...