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Washed red blood cells are red blood cells that have had most of the plasma, platelets and white blood cells removed and replaced with saline or another type of preservation solution. [ 1 ] [ 2 ] The most common reason for using washed red blood cells in transfusion medicine is to prevent the recurrence of severe allergic transfusion reactions ...
The direct Coombs test is used to detect antibodies or complement proteins attached to the surface of red blood cells. To perform the test, a blood sample is taken and the red blood cells are washed (removing the patient's plasma and unbound antibodies from the red blood cells) and then incubated with anti-human globulin ("Coombs reagent").
Serum is mixed and incubated at body temperature (37°C) with a 5% group O RBC suspension. (This step sensitizes or coats RBC with antibody) The sensitized RBC's are washed with PBS to remove any unbound antibody or interfering substances. The washed, sensitized RBC are then added to the monocyte monolayer tissue culture chamber slide.
Regardless of manufacturer, there are many types of cell processors. Cell processors are red cell washing devices that collect anticoagulated shed or recovered blood, wash and separate the red blood cells (RBC) by centrifugation or filtration such as the HemoClear filter. After, the washed RBCs can be returned to the same patient by reinfusion.
Blood compatibility testing is routinely performed before a blood transfusion.The full compatibility testing process involves ABO and RhD (Rh factor) typing; screening for antibodies against other blood group systems; and crossmatching, which involves testing the recipient's blood plasma against the donor's red blood cells as a final check for incompatibility.
The procedure of washing a patient's own red blood cells goes as follows: The patient's lost blood is collected and washed with a saline solution. The washing procedure yields concentrated washed red blood cells. The last step is reinfusing the packed red blood cells into the patient. There are multiple ways to wash red blood cells.
The washed blood can be labeled in the circular bag or transferred into a more traditionally shaped blood storage bag. Because the washing process creates an open system, red blood cells washed with this machine expire after 24 hours when kept at 1-6 °C. [2]
There are two main ways to salvage red blood cells. Cell processing and direct injection. Cell processors are red cell washing devices like the Cell Saver that collect anticoagulated shed or recovered blood, wash and separate the red blood cells (RBC) by centrifugation. Filtration devices like HemoClear microfilter, constitute the second major ...